Association of the Mr 58 , 000 Postsynaptic Protein of Electric Tissue with Torpedo Dystrophin and the M ; . 87 , 000 Postsynaptic
نویسندگان
چکیده
Margaret H. Butler$$, Karen DouvilleS, Amy A. MurnaneSll, Neal R. Kramarcy 11, Jonathan B. Cohen**, Robert Sealock11 , and Stanley C. Froehnerz $$ From the $Department of Bwchemistly, Dartmouth Medical School, Hanouer, New Hampshire 03755, the )I Department of Physiology, University of North Carolina, Chapel Hill, North Carolina 27599, and the **Department of Anatomy and Neurobiology, Washington University, St. Louis, Missouri 631 10
منابع مشابه
Dystrophin is a component of the subsynaptic membrane
A subsynaptic protein of Mr approximately 300 kD is a major component of Torpedo electric organ postsynaptic membranes and copurifies with the AChR and the 43-kD subsynaptic protein. mAbs against this protein react with neuromuscular synapses in higher vertebrates, but not at synapses in dystrophic muscle. The Torpedo 300-kD protein comigrates in SDS-PAGE with murine dystrophin and reacts with ...
متن کاملCloning and characterization of the human homologue of a dystrophin related phosphoprotein found at the Torpedo electric organ post-synaptic membrane.
Dystrophin is the protein product which is absent in Duchenne muscular dystrophy (DMD). In mammalian skeletal muscle, dystrophin is found in association with several integral and peripheral membrane proteins, forming a complex known as the dystrophin glycoprotein complex (DGC). In an expressed sequence tag (EST) database search to identify new dystrophin related genes, we isolated EST00891 whic...
متن کاملA novel 87,000-Mr protein associated with acetylcholine receptors in Torpedo electric organ and vertebrate skeletal muscle
To identify proteins associated with nicotinic postsynaptic membranes, mAbs have been prepared to proteins extracted by alkaline pH or lithium diiodosalicylate from acetylcholine receptor-rich (AChR) membranes of Torpedo electric organ. Antibodies were obtained that recognized two novel proteins of 87,000 Mr and a 210,000:220,000 doublet as well as previously described proteins of 43,000 Mr, 58...
متن کاملLocalization of dystrophin relative to acetylcholine receptor domains in electric tissue and adult and cultured skeletal muscle
Two high-affinity mAbs were prepared against Torpedo dystrophin, an electric organ protein that is closely similar to human dystrophin, the gene product of the Duchenne muscular dystrophy locus. The antibodies were used to localize dystrophin relative to acetylcholine receptors (AChR) in electric organ and in skeletal muscle, and to show identity between Torpedo dystrophin and the previously de...
متن کاملSyntrophin binds to an alternatively spliced exon of dystrophin
Dystrophin, the protein product of the Duchenne muscular dystrophy locus, is a protein of the membrane cytoskeleton that associates with a complex of integral and membrane-associated proteins. Of these, the 58-kD intracellular membrane-associated protein, syntrophin, was recently shown to consist of a family of three related but distinct genes. We expressed the cDNA of human beta 1-syntrophin a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2001